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KMID : 0525720120170030164
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Journal of Chitin and Chitosan
2012 Volume.17 No. 3 p.164 ~ p.168
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Inhibitory Effect of Deep Sea Water Containing Chitosan Oligosaccharides on Adipocyte Differentiation
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Shin Eun-Ji
Park Jung-Eun
Ha Byung-Geun
Cho Hyun-Jung
Lee Seung-Hee
Kim Mee-Kyung
So Myung-Suk
Shon Yun-Hee
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Abstract
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To determine effect of deep sea water (DSW) containing chitosan oligosaccharides (COS) on adipocyte differentiation, we performed Oil Red O staining to visualize and quantify lipid accumulation in differentiated 3T3-L1 adipocytes with the treatment of DSW alone or DSW containing COS. Both adipocyte differentiation and lipid accumulation were inhibited with the DSW alone or DSW containing COS in 3T3-L1 adipocytes in a dose-dependent manner. The amount of triglyceride (TG), an ester derived from glycerol and free fatty acids, in 3T3-L1 adipocytes was also decreased. Furthermore, expression of adipogenic genes: peroxisome proliferator-activated receptor gamma (PPAR-γ) and CCAAT/enhancer-binding protein alpha (C/EBP α), major adipogenic transcription factors, and glucose transporter 4 (GLUT4), a mature adipocyte marker, was decreased. These data suggest that DSW containing COS is more effective than DSW alone in inhibitory effect on adipocyte differentiation.
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KEYWORD
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Chitosan oligosaccharide, Deep sea water, Adipocyte differentiation, PPAR-γ, C/EBP α
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